Comparison of Neurospora crassa and Neurospora sitophila for phytase production at various fermentation temperatures

Heterokaryosis in Neurospora sitophila.

ETEROKARYOSIS has been extensively used as a major tool in formal genetic analysis of both Mendelian and non-Mendelian genes and in establishing gene-enzyme relationships. So far it is the only way to perform genetic anslysis of somatic cells in tissue culture (EPHRUSSI 1965; MINTZ and BAKER 1967). These aspects of heterokaryosis have been reviewed elsewhere ( JINKS 1965; FINCHAM 1966; DAVIS 19...

Biotransformation of Hydrocortisone by Neurospora crassa

The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...

MOLECULAR ANALYSIS OF THE SULFUR REGULATORY CIRCUIT OF NEUROSPORA CRASSA

The sulfur regulatory circuit of the filamentous fungus, Neurospora crassa, consists of a set of unlinked structural genes which encode sulfur catabolic and two major regulatory genes which govern their expression. The cys-3 regulatory gene encode a transacting regulatory protein which activates the expression of cys-14 and ars, whereas the other regulatory genes Scon-l and Scon-2 appear to...

Quelling in Neurospora crassa.

Heterocaryosis in Neurospora Crassa.

T IS possible by means of X-ray or ultraviolet treatment to produce mutant I strains of Neurospora crassa that are deficient in their abilities to synthesize specific vitamins, amino acids, or other essential substances (BEADLE and TATUM 1941; TATUM and BEADLE 1g4zb). It occurred to us that it might be possible to determine whether the mutant genes concerned in such haploid strains are recessiv...